Very much so, and the difference is usually crew model and operator culture rather than anything on the spec sheet. Two ships of similar size and similar cabin counts can produce completely different weeks. Some attract a sociable, younger crowd who end up in the bar playing board games with the guides. Others run more formal, with higher-end food and a quieter rhythm.
There's an operational side to this that's invisible when you're comparing brochures. Operators who staff their own crew, including able seamen and crane operators, can move faster when the weather demands it. That occasionally means running a 4am or 5am landing to get ahead of an incoming system. It's rare, a couple of times a season, but it's possible for some operators and not for others. Operators using contracted crew have less of that flexibility.
None of this is a ranking. All of these ships deliver a strong expedition experience. It's about matching you to a room you'll enjoy being in for two weeks.
This is exactly the kind of thing that doesn't show up in a spec comparison and does show up on day three. Ask us what a given ship is like, and we'll tell you honestly, including when it isn't your crowd.